Nanolive’s CX-A extends the exploration of living cells from single cells to cell populations without compromising on the highest precision and resolution proper to all Nanolive products. It enables scientists to investigate macro cellular dynamics like cell health, proliferation, movement and function as well as micro organelle dynamics and interactions e.g. mitochondrial network characterization.
The CX-A can image living cells for a virtually limitless amount of time. Thanks to its unique harmless way of cell preparation and observation, hundreds of images can be collected each hour, transforming endpoint assays to continuous analysis, for days or weeks, while cells remain unperturbed in a physiologically controlled environment. Users can freely define the perfect imaging regimen for their cells without worrying about phototoxicity or photobleaching.
These unique properties of Nanolive’s CX-A system enable users to continuously monitor their precious, most-sensitive cells and to study their finest behaviors without missing anything.
Nanolive’s completely redesigned intuitive user-interface enables first-time users to set-up experiments in just a few minutes and walk away, while the CX-A automatically collects the images. In addition to improving data significance, multiple imaging regimens can be programmed within the same plate allowing users to run different applications in parallel. A real-time preview allows the user to navigate through the data at any time while offering an overview of the experimental protocol. The 3D dataset output can then be exported with just a few clicks to multiple data formats for analysis.
Illumination source: |
Class 1 laser low power (λ=520 nm, sample exposure 0.2 mW/mm2) |
Resolution |
3D Holotomography (HT): x,y: 200 nm; z: 400 nm |
Field-of-view (FoV) |
Single FoV* / 2x2 FoV / 3x3 FoV |
Channels |
HT: Up to 8 organelles simultaneously |
Imaging modalities |
Automated: 3D HT | 3D HT + Epifluorescence | 4D HT time-lapse | 4D HT + Epifluorescence time-lapse |
Sample holder |
Nanolive’s 96 well plates, designed for high precision imaging with optical quality glass bottom and lid |
Autofocus |
High precision label-free autofocus for stable long-term observations in all imaging modalities |
Incubator stage-top |
Tokaihit stage top incubator: CO2 concentration range: 5% - 20% (±0.1%); Humidity: ~ 95%; Sample temperature: 30-40°C (±0.3°C) |
Camera |
USB 3.0 CMOS Sony IMX174 sensor / Quantum Efficiency (typical) 70 % (at 545 nm) / Dark Noise (typical) 6,6 e¯ / Dynamic Range (typical) 73,7 dB |
Microscope Objective |
Dry objective / 60x magnification / NA 0.8 |
Weight |
~30Kg |