Volocity Acquisition

Volocity® Acquisition is designed for high speed, 3D image capture. It provides a flexible capture interface to a wide range of cameras, microscopes and associated hardware, so that you can select the most suitable hardware items for your application without compromise. Capture protocols can be quickly constructed using the clear and easy to use Acquisition dialogue – from simple 2D image capture to multi-channel 4D experiments.

Flexible protocol design for all your imaging experiments:
Tailor your experiment to the biology: Volocity Acquisition is configurable with different hardware, and once set up in preferences you can construct complex and varied acquisition and analysis protocols without the need for complex macro writing skills.
Image acquisition made easy:
You can view your sample in the Video Preview before  you start to capture images. You have the option to apply a color look-up table and Auto Contrast  to both  the Video Preview and to the images as they are acquired. Auto Contrast allows you to set a shorter camera exposure without reducing the apparent brightness of the image – ideal for fast focusing or very rapid image acquisition. Customize the hardware interface controls on the Video Preview palette  to hide items you don’t use, so that navigation is easier. Once you have set up your acquisition hardware for a particular task, you can save the settings  in the Light Path Manager. Properties that  can be saved include filter position, objective turret, camera exposure and binning for use with a particular fluorochrome. This means that when you want to use the same settings again, you can simply click on the appropriate light manager button and they will be restored, saving you time and providing consistency.

Time lapse imaging with confidence:
 When using Volocity to acquire images over time, you don’t have to wait until the end of the experiment to see the results; you can review the data as soon as the first time point has been captured, so that you can be sure that your protocol is working correctly right away. This means that you don’t waste valuable hours acquiring a data set that has failed to develop as expected. 

Multi-parameter protocols:
Protocols that  involve scanning  multiple points on a well plate or slide are easy to configure by first creating a Well Overlay of the selected or random points from which you wish to acquire images. Well overlays can be saved for reuse. By selecting the XY Stage in the Acquisition Setup dialog, images will be captured at the points set in the well overlay. The acquisition protocol can include image capture at multiple wavelengths, multiple time points and in 3D at each point you have selected, for complete flexibility. Supports XYZ stitching for high resolution imaging in large fields of view.

Volocity 3D Acquisition Solutions:
Volocity Acquisition drives a range of 3D imaging solutions, designed for applications ranging from multi-channel fixed cell imaging to 4D live cell confocal imaging using spinning disk technology. We can provide a complete hardware and software system, or drive your existing hardware where appropriate, for a cost effective and flexible approach to your imaging requirements.
FRAP and Ratio Acquisition:
Volocity Acquisition can be used to acquire and analyze FRAP and ratiometric  imaging experiments,  by adding  the optional  FRAP Plugin and Ratio Plugin to your system. Volocity Quantitation must be included in the Volocity configuration in order to perform analysis.

Volocity, a family of integrated software products that can be used independently for image acquisition or analysis, or grouped together to provide a complete range of cellular imaging tools.

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