Mosaic System

Mosaic is the perfect tool for active illumination applications such as Optogenetics, optophysiology, photobleaching/activation and uncaging. This patented combination of digital mirror device used with a microscope delivers high speed targeting of complex light patterns to your sample. For optogenetics you can even combine complimentary activating and inhibitory wavelengths at the same time for tight spatial target protein control

Mosaic is a patented instrument platform built around MEMS Digital Mirror Devices (DMD). DMDs were developed at Texas Instruments in 1987 and are now in widespread use in digital projectors and other display devices. The DMD comprises an array of individually addressable micro-mirrors that can be switched "on and off" (tilted) with MEMS "hinge" elements. DMD arrays contain hundreds of thousands to millions of micro-mirrors.

Mosaic exploits DMD in a proprietary programmable platform, integrated with scientific light sources including lasers, LEDs and arc lamps, and operates from 360 to 800 nm. It is offered with a range of high performance microscope adapter optics and can be integrated with CLSM, spinning disk and wide field imaging modalities.

High speed frame switching (5000 Hz) makes Mosaic suitable for many dynamic applications including bleaching, uncaging, photoswitching, optogenetics and constrained illumination. Variable intensity distributions can be achieved by rapid gating of mirror patterns. Mosaic has a unique capability to illuminate in parallel an arbitrary number of complex regions (sometimes called “zero delta t”) that sets it apart from galvo-based devices and makes it especially attractive for uncaging, photoswitching and light activation. It is a unique tool for the study of optically stimulated intra and inter-cellular activity in neuroscience and physiology, as well as for function-structure studies with photoswitching fluorescent proteins. Mosaic uses dichroic coupling to the microscope light path and is therefore capable of simultaneous stimulation and imaging.

Optogenetics Mosaic System

Mosaic is the perfect tool for active illumination applications such as Optogenetics, optophysiology, photobleaching/activation and uncaging. This patented combination of digital mirror device used with a microscope delivers high speed targeting of complex light patterns to your sample. For optogenetics you can even combine complimentary activating and inhibitory wavelengths at the same time for tight spatial target protein control

Mosaic is a patented instrument platform built around MEMS Digital Mirror Devices (DMD). DMDs were developed at Texas Instruments in 1987 and are now in widespread use in digital projectors and other display devices. The DMD comprises an array of individually addressable micro-mirrors that can be switched "on and off" (tilted) with MEMS "hinge" elements. DMD arrays contain hundreds of thousands to millions of micro-mirrors.

Optogenetics

Optogenetics is a rapidly growing technique used across an ever broadening array of research fields. Investigators now genetically modify many signalling pathway elements to enable precise and spatially targeted light control of biology down to a single cell or sub-cellular level. Mosaic’s original and patented combination of DMD (micro-mirror array) with microscopy delivers the perfect tool for optophysiology.

Neuroscience, probably the largest research field using targeted light, commonly uses an upright microscope. Mosaic Infinity has been designed to couple simply to most upright scopes


Flexible patterning for complex networks and dynamics
High-speed (5KHz) to meet biological demands
Simultaneous dual-colour illumination (excite & inhibit)
Supports several light source types (e.g. laser, LED, Hg)

Can be used with p-CLAMP™
Also capable of uncaging and photo-bleaching/activation

We recommend the X-Cite XLED1 for use in Optogenetics. 

Micropoint System

MicroPoint provides a flexible and field-proven tool for photo-stimulation. Supplied with a patented compact, pulsed nitrogen pumped tunable dye laser it is capable of ablation, bleaching and uncaging over a wavelength range of 365 to 656 nm.

Product summary

MicroPoint provides a flexible and field-proven tool for photo-stimulation. Supplied with a patented compact, pulsed nitrogen pumped tunable dye laser it is capable of ablation, bleaching and uncaging over a wavelength range of 365 to 656 nm.

Broad wavelength range and energy control allow MicroPoint to be optimized for a wide range of scenarios. More than 20 wavelengths can be utilized with available dye resonator cells, while appropriate dichroic filter sets enable simultaneous imaging and photo-stimulation of the specimen. MicroPoint is supplied with a UV-Vis imaging quality Epi illumination adapter for both current and previous generation microscopes from Leica, Nikon, Olympus and Zeiss.

MicroPoint systems consist of a wavelength tuneable pulsed laser, coupling optics, beam steering optics, a microscope adapter, a selection of beam splitters and interference filters, and a motorized or manually driven optical attenuator to adjust spot size and power. There are three MicroPoint versions available:

MicroPoint Manual

Angular and spatial alignment of the illumination at the sample target is manually controlled via a 2-axis joystick. This manual version can be upgraded in-situ to provide galvo or bluetooth control.

MicroPoint Galvo

Galvanometer based beam steering is provided through PC control, enables precise and repeatable laser ablation and/or illumination in synchronization with other experiment parameters.

MicroPoint Bluetooth

In laser marking or circuit isolation applications a computer is often unnecessary, the advanced and automated features of the MicroPoint can be controlled through the handheld PDA interface.