iLAS FRAP


The iLas MODULAR system is a unique multi-application device that offers complete control over any laser illumination. Its evolutive design allows researchers to choose and simultaneously combine:

360° TIRF imaging
Single molecule imaging
oblique illumination imaging
FRAP
Photo-activation
Laser ablation
Micro-patterning
Fast spinning disk confocal imaging
Widefield imaging


Targeted laser illumination (FRAP/PA/Ablation)

Localized laser action techniques such as Fluorescence Recovery After Photobleaching (FRAP, FLIP), photoactivation, uncaging, photo-ablation are very powerful tools to photo-manipulate tissues or to analyze intracellular dynamics of proteins and other macromolecular complexes. For example, FRAP permits perturbation of the steady state fluorescence distribution by bleaching fluorescence in selected regions. After the bleaching step, researchers can observe and analyze how the fluorescence distribution returns to the same or a different steady state, giving appraisal on the spatiotemporal half life of molecule of interest within one particular site of a living sample. Photo-activation or photo-conversion make use of photo-convertible probes, allowing morphological “pulse and chase” experiments.

The iLas² system provides an easy-to-use interface to manage the lasers, set-up ROIs and plan the experiment. In order to lighten the acquisition process and enhance steering speed, iLas² is driven by its own electronic. Vectorial scanning and live action mode provide the ability to measure the fastest phenomena. The user can bleach fast-moving structures and analyze their recovery as they continue to move with the help of tracking algorithm.

From more information please contact: This email address is being protected from spambots. You need JavaScript enabled to view it.